Test Name:Prothrombin Time (PT)
Test: Prothrombin Time (PT)
Sample Type: 4 ml. (3 ml. Minimum) PPP (Platelet Poor Plasma) from 2 Sodium Citrate tubes prepared within 1 hr of collection. Aliquot PPP in 2 transport vials and FREEZE.
Instruction: Preparation of PPP :12 -14 Hrs. (Overnight) fasting is preferred. Centrifuge the blood sample at 3000rpm for 10 minutes within 1 hr. of collection to separate the plasma. Transfer the Plasma obtained to a clean plastic tube using plastic tipped pipette avoiding buffy coat & centrifuge it again at 3700 rpm & finally transfer the PPP into fresh, clean & labelled plastic tubes which contains <10,000 cells/cu. mm. DULY FILLED COAGULATION REQUISITION TO BE ATTACHED
TAT: Same Working Day by 7pm